Test Category: Biochemistry
Investigation Name:Insulin & C-Peptide
Accreditation:UKAS Accredited ISO15189:2022, on scope
Intro:
Primary indication: The investigation of spontaneous (non-diabetic) hypoglycaemia.Note: requests are vetted. Contact the duty biochemist on 01902 695 290 or rwh-tr.bcpsdutybiochemist3@nhs.net if urgent analysis is required.
Pathology Laboratory:Clinical Chemistry/Biochemistry
Requestable Seperately?Yes
Units:pmol/L
Minimum Sample Volume:0.5 mL for each
Expected Turnaround Time:1 week (urgent analysis possible - contact the duty biochemist on 01902 695290/rwh-tr.bcpsdutybiochemist3@nhs.net to arrange)
Test Code:INCP
Sample Type:
Serum (gold top/red top) preferred, lithium heparin (green top) accepted (paediatrics only)Complex Reference Range:Contact Duty Biochemist for interpretation
Collection Conditions / Other Information:Only of value in the investigation of proven spontaneous (non-diabetic) hypoglycaemia.
Must be accompanied by paired glucose sample.
Must be delivered to CLINICAL CHEMISTRY immediately after collection for rapid processing of sample.
LAB INSTRUCTIONS: Separate into aliquot within 6 hours and store serum at -20°C and Transport frozen.
Referred to Another Laboratory?No
EQA Scheme:UKNEQAS Guildford Peptides scheme
Further information:Assay limitations:
Haemolysed samples are not suitable for analysis due to in vitro degradation of insulin
The Abbott assay has the following cross-reactivity with these insulins:
>80%:
Actrapid, Humulin (S/I), Insulatard, Hypurine neutral porcine, Novorapid (aspart), Humalog (lispro), Lantus (glargine), Hypurine isophane bovine, Hypurine protamine zinc
50-100%:
Levemir (determir)
25-30%
Tresiba (degludec, Hypurine neutral bovine
5-10%: Apidra (glulisine), Hypurine isophane porcine
Methodology:Abbott Alinity i
Site Sample Tested:Russell's Hall Hospital, RWT
Contact Number:01902 307 999 Ext 88266
