< Return to all Tests

Test Category: Immunology

Investigation Name:Myeloperoxidase Antibody (MPO)

Alias or Abbreviation:MPO, ANCA

Intro:

Indication:
Microscopic polyangiitis (MPA)

Further information:
In accordance with the revised 2017 international consensus on testing of ANCAs, ANCA requests should be confined to patients with a high pre-test probability of small vessel vasculitis, if misleading 'false positives' in a range of non-vasculitic disorders are to be avoided. Indications are:
• Glomerulonephritis, especially rapidly progressing glomerulonephritis
• Pulmonary haemorrhage, especially pulmonary renal syndrome
• Acute pulmonary-renal syndrome
• Cutaneous vasculitis with systemic features
• Multiple lung nodules
• Chronic destructive disease of the upper airways
• Long-standing sinusitis or otitis
• Granulomatous nasopharyngeal lesions
• Subglottic tracheal stenoses
• Mononeuritis multiplex, or other peripheral neuropathy
• Retro-orbital mass
• Scleritis

p-ANCA/MPO antibodies are found most often in patients with microscopic polyarteritis but may also be seen in GPA and EGPA. P-ANCA/MPO antibodies may also be seen in idiopathic or vasculitis-associated necrotizing crescentic glomerulonephritis.

30-40% of patients with Goodpasture syndrome have anti-MPO along with anti-GBM. these patients are generally older and have less severe renal disease than those with anti-GBM alone.

Note 'false positives' may occur in diseases which may mimic systemic vasculitis e.g. infective endocarditis, tuberculosis, non-Hodgkin's lymphoma, acquired immune deficiency syndrome.

p-ANCA/MPO may also be present non-specifically in other autoimmune disorders, such as systemic lupus erythematosus, rheumatoid arthritis, and Sjögren syndrome.

NOTE: Results >3.5 IU/mL are reflexed for ANCA test on new patients only.

For urgent requests, contact the laboratory directly.

Pathology Laboratory:Immunology laboratory

Requestable Seperately?No

Minimum Sample Volume:1mL

Expected Turnaround Time:3 Weeks

Test Code:ANCA

Sample Type:

Serum Gel Tube (Gold top)/ Plasma

Complex Reference Range:<3.5 IU/mL = Negative

Collection Conditions / Other Information:1st class post or Transport

Referred to Another Laboratory?No

Storage Requirements:Ambient temperature

NPEX / PDF Reporting Available:Yes

EQA Scheme:Yes

Further information:Orderable in panel with PR3

Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net

Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net

Methodology:Fluoroenzymeimmunoassay

UKAS Reference Number:9580 (recommended)

Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton

Contact Number:01902 695 279 or Ext 85279