Test Category: Immunology
Investigation Name:Epidermal Antibodies Screen (Epidermal Intercellular antibodies, Epidermal Basement antibodies)
Alias or Abbreviation:Skin, Pemphigus, Pemphigoid
Intro:
Indication:Autoimmune skin diseases.
Further information:
The blistering skin diseases where IIF is used are:
• Pemphigus Vulgaris (PV)
Serum antibodies directed against the cell surface of epidermal keratinocytes are found in 90% of patients with Pemphigus and correlate with disease activity.
• Bullous Pemphigoid
Serum antibodies directed against the basement membrane are present in 70% of patients. Antibody levels do not reflect disease activity and antibody tends to persist even following successful therapy. Antibodies are uncommon in normal individuals or in other skin diseases.
Pathology Laboratory:Immunology laboratory
Requestable Seperately?Yes
Minimum Sample Volume:1mL
Expected Turnaround Time:3 weeks
Test Code:EPID
Sample Type:
Serum Gel Tube (Gold top)Complex Reference Range:Positive/ Negative
Collection Conditions / Other Information:1st class post or Transport
Referred to Another Laboratory?No
Storage Requirements:Ambient temperature
NPEX / PDF Reporting Available:Yes
EQA Scheme:Yes
Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net
Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net
Methodology:Indirect Immunofluorescence
UKAS Reference Number:Awaiting assessment
Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton
Minimum Request Interval (MRI):180 days (6 months) over 16y only
Contact Number:01902 695279 or Ext 85279
