Test Category: Immunology
Investigation Name:Myeloperoxidase Antibody (MPO)

Alias or Abbreviation:MPO, ANCA
Intro:
Indication: Microscopic polyangiitis (MPA) Further information: In accordance with the revised 2017 international consensus on testing of ANCAs, ANCA requests should be confined to patients with a high pre-test probability of small vessel vasculitis, if misleading 'false positives' in a range of non-vasculitic disorders are to be avoided. Indications are: • Glomerulonephritis, especially rapidly progressing glomerulonephritis • Pulmonary haemorrhage, especially pulmonary renal syndrome • Acute pulmonary-renal syndrome • Cutaneous vasculitis with systemic features • Multiple lung nodules • Chronic destructive disease of the upper airways • Long-standing sinusitis or otitis • Granulomatous nasopharyngeal lesions • Subglottic tracheal stenoses • Mononeuritis multiplex, or other peripheral neuropathy • Retro-orbital mass • Scleritis p-ANCA/MPO antibodies are found most often in patients with microscopic polyarteritis but may also be seen in GPA and EGPA. P-ANCA/MPO antibodies may also be seen in idiopathic or vasculitis-associated necrotizing crescentic glomerulonephritis. 30-40% of patients with Goodpasture syndrome have anti-MPO along with anti-GBM. these patients are generally older and have less severe renal disease than those with anti-GBM alone. Note 'false positives' may occur in diseases which may mimic systemic vasculitis e.g. infective endocarditis, tuberculosis, non-Hodgkin's lymphoma, acquired immune deficiency syndrome. p-ANCA/MPO may also be present non-specifically in other autoimmune disorders, such as systemic lupus erythematosus, rheumatoid arthritis, and Sjögren syndrome. NOTE: Results >3.5 IU/mL are reflexed for ANCA test on new patients only For urgent requests, contact the Laboratory directlyRequestable Seperately?No
Minimum Sample Volume:1mL
Test Code:ANCA
Sample Type:
Serum Gel Tube (Gold top)/ PlasmaComplex Reference Range:<3.5 IU/mL = Negative
Collection Conditions / Other Information:1st class post or Transport
Referred to Another Laboratory?No
Storage Requirements:Ambient temperature
NPEX / PDF Reporting Available:Yes
EQA Scheme:Yes
Further information:Orderable in panel with PR3
Lead Contact Details:rwh-tr.bcpsimmunologyclinicalqueries@nhs.net
Email Address For Chasing Results:rwh-tr.ImmunologyLab@nhs.net
Methodology:Fluoroenzymeimmunoassay
UKAS Reference Number:9580 (recommended)
Site Sample Tested:Immunology, New Cross Hospital, Wolverhampton